Estimation of acute toxicity of bioinsecticide Turinbash-G for honey bee <i>Apis mellifera mellifera L.</i>

Introduction . Pesticide poisoning is one of the main causes mass death bee colonies observed in different countries world. However, at present, abandoning use plant protection products not possible, since insects and crop weeds are responsible for loss least half world's food supply. An increase insect pest activity due to global warming will lead even greater losses. All this dictates need a wider introduction biological methods protection. Bioinsecticides based on entomopathogens can become an alternative chemicals that have pronounced toxic effect nontarget organisms. Methods. The object study was honey Apis mellifera L Carpathian breed, subjected oral contact exposure Turinbash-G, active ingredient which bacterium Bacillus thuringiensis subsp aizawai strain 12K thuringiensis, VNIIVEA-177. As guide, work used «Methodological recommendations assessing potential hazard pesticides honeybees», approved by Russian Academy Agricultural Sciences Department Veterinary Medicine Ministry Agriculture Federation, as well GOST 33038-2014 33039-2014 «Mеthods testing chemical posing danger environment.» In course studies, acute toxicity substance Turinbash-G. Results. Studies revealed signs Turinbash-Zh bioinsecticide bees both with drug. maximum mortality rate experimental groups 96 hours after end studied drug 16.7%. LD 50 determining — above value 1·10 6 CFU/bee, LK 100 ml/l; 5 ml/l.